Physiological characterization of a mouse model of cachexia in colorectal 1 liver metastases

Physiological characterization of a mouse model of cachexia in colorectal 1 liver metastases 2 3 Kate T. Murphy, Adam Struk, Cathy Malcontenti-Wilson, Christopher Christophi and 4 Gordon S. Lynch 5 6 Basic and Clinical Myology Laboratory, Department of Physiology, The University of 7 Melbourne, Victoria 3010, Australia 8 Liver and Pancreas Research Group, Department of Surgery, The University of Melbourne, 9 Austin Health, Heidelberg 3084, Australia 10 †Recently deceased 11 12 Running Head: Cachexia in colorectal liver metastases 13 14 Author Contributions: K.T.M. conceived and designed the study, performed passage 15 injections, biochemical and molecular biological assays, analyzed the data and prepared and 16 revised the manuscript. A.S. performed passage injections, animal monitoring, dissections, 17 grip strength and rotarod analyses, and immunohistochemistry. C.M.W. performed passage 18 injections and intrasplenic injections. C.C. and G.S.L. participated in the design and 19 coordination of the study and helped draft and revise the manuscript. All authors read and 20 approved the final manuscript. 21 22 Correspondence to: Kate T. Murphy, Ph.D. 23 Basic and Clinical Myology Laboratory 24 Department of Physiology 25 The University of Melbourne 26 Victoria 3010 Australia 27 Ph: +61 3 8344 0065 28 Fax: +61 3 8344 5818 29 Email: [email protected] 30 31 Articles in PresS. Am J Physiol Regul Integr Comp Physiol (March 13, 2013). doi:10.1152/ajpregu.00057.2013